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Localization of epidermal A- and N-SMase activities. Hairless mice were injected intradermally with staphylococcal exfoliative. After 2 hours, outer and inner epidermal layers were isolated and (a) A-SMase (*P < 0.0001, n = 6) and (b) N-SMase (NS, n = 6) assays were performed. (c) SC samples were obtained by tape stripping. Cells from the outer (first tape strip) and the inner (fifth tape strip) were eluted in buffer A, and A-SMase activity was determined (P < 0.0001, n = 10).
