Table 2.

QCMF-significant essential sites in the human GCK protein, which are nearby either nsSNPs or strictly conserved sites

QCMF-significant	Nearby nsSNPs or strictly	Reference
essential sites	conserved sites
M37	36 s ,39 s ,40 s	[38,39,43,51]
S76	147 c
L79	78 c ,80 c ,150 c	-
T82	81 c	-
N83	81 c ,108 s ,110 s	[43,51]
V86	85 c ,106 s	[38]
S127	130 s	[40]
F148	147 c ,150 c,s	[38,39,43,51]
F152	150 c,s , 151 c	[39,43,51]
P153	154 s	[39]
H156	154 s	[39]
A176	119 s ,175 s	[43]
G178	164 c
N180	162 s ,182 s	[38,39,43],
L185	182 s ,188 s	[39,43,51]
A201	147 c ,453 c
M202	147 c ,203 s	[43]
A232	223 s ,231 c	[39,40,51]
C233	223 s ,234 c ,235 c	[39,40,51]
V253	234 c ,254 c
F260	257 s ,258 c ,259 s ,261 s	[39,43]
L271	274 c
V277	274 c ,278 c ,279 s	[43]
S281	278 c ,279 s	[43]
Y297	291 c ,295 c ,299 c , 300 s	[43]
M298	295 c ,299 c ,300 s	[43]
T332	295 c ,299 c
V374	377 c
A378	377 c ,382 s	[43]
A379	377 c ,382 s	[43]
S383	382 s ,385 s	[43]
A384	382 s ,385 s	[43]
A387	385 s	[43]
S388	385 s ,392 s	[38,43]
V412	226 s ,227 c ,410 c ,414 s ,416 s	[40,43]
F419	416 s	[40]
E443	444 c ,445 c ,447 s	[39]
G446	444 c ,445 c ,447 s ,448 c ,449 c	[39]

^s : non-synonymous snp site, ^c : strictly conserved site.