Figure 4.

Wnt activation-mediated differentiation mainly affects CD133⁺ GBM-derived cells. (a and b) BAT-lux (a) and CBF1-luc (b) reporter analysis of CD133⁺ or CD133⁻ sorted GBM cells treated with Wnt3a or transfected with CA-β-catenin at 2% O₂. Mean of four tumours±S.E.M., n=2 for each tumour. (c and d) Representative immunofluorescence images of CD133^± sorted GBM cells treated with Wnt3a for 96 h and stained for Nestin (green)/β-III-tubulin (red) (c) and relative quantification (d). Bar=100 μm. (e and f) Representative immunofluorescence images of same cells as (c) and (d) stained for Ki67 (red) (e) and relative quantification (f). Bar=100 μm. Mean of four tumours±S.E.M., n=3 for each tumour. *P<0.05, **P<0.01, ***P<0.001