Figure 7.

Neuronal differentiation of grafted GBM cells is dependent on zebrafish Wnt ligands. (a and b) hsp70l:dkk1-GFP xeno-transplanted larvae at 4, 48 and 96 hpi stained for Nestin (green)/β-III-tubulin (red) (a) and Ki67 (green)/β-actin (red) (b). Bar=40 μm. (c and d) Bar graphs showing relative quantification of images described in (a) and (b). Mean of six tumours injected±S.E.M., n=50 for each experimental group. (e) Survival graph of post-transplanted hsp70l:dkk1-GFP zebrafish compared with sham±heat shock, bred in the same conditions. Mean of six tumours injected±S.E.M., n=50 for each experimental group. ***P<0.001