Figure 3.

AT_1A receptor mRNA expression in cell populations isolated from spleen. AT_1A receptor mRNA expression in spleen assessed by RT-PCR. Total RNA was isolated, and RT-PCR was performed using primers specific for the AT_1A receptor (7). The PCR products were size fractionated on an agarose gel that was stained with ethidium bromide. The 457-bp PCR product is easily seen in samples from isolated splenic T cells (lane 2), B cells (lane 3), and macrophages (lane 4), or unseparated splenocytes (lane 5) from a wild-type mouse. No product was detected in RNA isolated from splenocytes of an AT_1A receptor–deficient mouse (lane 6).