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. 1999 Dec 15;104(12):1739–1749. doi: 10.1172/JCI7779

Figure 1.

Figure 1

Expression of chemokine receptors on bone marrow CD34+ BMMNC. BMMNC were isolated from bone marrow aspirates of healthy donors by Ficoll-gradient centrifugation and then stained with mAbs against CD34 antigen (FITC) and chemokine receptors (PE). (a) Forward (FSC) and side scatter (SSC) analysis of BMMNC. Lymphocyte region is defined by R1. (b) Analysis of cells dual-labeled with FITC anti-CD34 mAb and PE anti-CXCR4 mAb. (c) Analysis of cells dual-labeled with FITC anti-CD34 mAb and PE anti-CCR5 mAb. Data from at least 5 different donors were analyzed with similar results. Data from a representative donor is presented.