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Intracellular staining for RANTES in CD34+ cells that were isolated using MiniMACS beads and then permeabilized. (a) Forward and side scatter analysis of CD34+ cells isolated using MiniMACS beads. (b) Iso-PE staining. (c) PE anti-RANTES mAb. (d) PE anti-RANTES mAb in CD34+ cells stimulated for 24 hours with IFN-γ (1,000 U/mL). Data from 3 different donors were analyzed with similar results. Data from a representative donor are presented.
