Figure 12.

PB-1 cells infected with R5 HIV (Ba-L) in the presence of conditioned medium (CM) collected from CD34⁺ cell cultures, conditioned medium that contained a cocktail of neutralizing Abs (50 μg/mL α–MIP-1α, 50 μg/mL α–MIP-1β, and 10 μg/mL α-RANTES) (CM with Ab), and control culture medium (SM) that was mock cleared with an equal amount of nonreactive goat Ig (110 μg/mL). Results are representative of 3 separate experiments and are presented as mean p24 (ng/mL) of triplicate measurements. The multiplicity of infection for both viruses was 0.02. There was a significant decrease in Ba-L p24 production at day 5 and day 10 in the cultures when PB-1 cells were infected in the presence of supernatant from CD34⁺ cell cultures (P < 0.001). The decrease in Ba-L p24 production was only partially abrogated by preclearing conditioned medium from CD34⁺ cells with neutralizing mAbs.