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. 1999 Dec 15;104(12):1739–1749. doi: 10.1172/JCI7779

Figure 2.

Figure 2

Intracellular expression of CCR5 on bone marrow CD34+ BMMNC. BMMNC were isolated from bone marrow aspirates of healthy donors by Ficoll-gradient centrifugation. Cells were permeabilized before staining with mAbs against CD34 antigen (FITC) and CCR5 receptor (PE). (a) Forward (FSC) and side scatter (SSC) analysis of permeabilized BMMNC. Lymphocyte region is defined by R1. (b) Iso-PE staining. (c) FITC anti-CD34. (d) Analysis of cells dual-labeled with FITC anti-CD34 mAb and PE anti-CCR5 mAb. Data from at least 3 different donors were analyzed with similar results. Data from a representative donor is presented. We obtained similar data by using 3 other mAbs (clones 2D7 and 549 from R&D Systems Inc., and CTC5 from Protein Design Laboratories Inc.) for CCR5 detection.