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Intracellular staining for MIP-1α in CD34+ cells isolated using MiniMACS beads and subsequently permeabilized. (a) Forward and side scatter analysis of CD34+ cells isolated by MiniMACS beads. (b) Iso-PE staining. (c) PE anti–MIP-1α mAb. (d) MIP-1α detection has been specifically blocked by addition of MIP-1α. Data from 3 different donors were analyzed with similar results. Data from a representative donor are presented.
