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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Immunity. 2011 Dec 8;35(6):883–896. doi: 10.1016/j.immuni.2011.11.010

Fig. 7. MYSM1 interacts with the transcription factor E2A and BRM.

Fig. 7

a. Sequential two-step ChIP assays of WT BM Lin-progenitors were performed, showing the recruitment of the endogenous E2A and MYSM1 to the Ebf1 promoter from one of two independent experiments. The relative binding was defined by determining the immunoprecipitation level (ratio of the amount of immunoprecipitated DNA to that of the input sample) and then comparing to corresponding 1st ChIP or 2nd ChIP control IgG immunoprecipitation level, which was set as 1.0. **p < 0.01.

b & c. Endogenous BRM and BRG1 coimmunoprecipitated with MYSM1. Cell lysates from HEK293T cells transfected with a pCMV-Flag-Mysm1, or pCMV-FLAG expression plasmid were incubated with an anti-Flag antibody, and immunoprecipitated proteins were analyzed by a BRM (b) or BRG1 (c) antibody. Ten percent of the input was loaded.

d. Sequential two-step ChIP assays of WT BM Lin-progenitors were performed from one of two repeated experiments. **p < 0.01.