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Shift in the cytokine profile from a Th1 to a Th2/Th3 type. LNCs from rats treated at the acute phase of EAMG with Hα1-205 or OVA were cultured for 2 days in the presence of AChR, and mRNA was prepared. The mRNA expression level of cytokines (and of β-actin as control) was determined by PCR-ELISA, and the data are expressed as the relative value of the Hα1-205–treated group compared with the OVA-treated group.
