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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Methods Mol Biol. 2007;391:127–144. doi: 10.1007/978-1-59745-468-1_10

Fig. 7.

Fig. 7

Bubble traps in the flow cell apparatus are sequentially filled by turning a bubble trap stopcock to the “on” position. Each bubble trap cylinder is filled approximately half full with medium, and then the bubble trap stopcock is returned to the “off” position. This process is repeated for each of three bubble traps per flow cell.