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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Mol Cell Biochem. 2014 Jan;385(0):145–157. doi: 10.1007/s11010-013-1823-3

Fig. 2.

Fig. 2

A Validation of a BMP-2 reporter assay for screening activity of TAT-LMP-1. The dose-dependent induction of luciferase activity by BMP-2 in C2C12 cells transfected with BMP-specific and Smad1-driven 9×GCCG reporter plasmid is shown. The reporter responded dose dependently to BMP-2. B Potentiation of BMP-induced response by LMP-1. A dose response to BMP-2 by the reporter construct, and clear potentiation of BMP-2 responsiveness by purified recombinant TAT-LMP-1 protein is observed. Luciferase activities were determined in triplicate