Fig. 6.

Mutation of the Smurf1-interaction motif or the Jab1-interaction motif in LMP-1 results in loss of binding to the respective target proteins. Mutation of the Smurf1-interacting motif in LMP-1 (LMP-1ΔSmurf1) resulted in loss of binding to Smurf1 without affecting its binding affinity for Jab1. Similarly, mutation of the Jab1-interacting motif (LMP-1ΔJab1) resulted in loss of binding of LMP-1 to Jab1 without affecting its interaction with Smurf1. A double mutant (ΔΔ Smurf1–Jab1) lacking the required motifs for Smurf1 and Jab1 fails to bind these target proteins