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Knockdown of Jab1 by siRNA elevates Jab1 mRNA levels in C2C12 cells. The C2C12 cells were transfected with control or Jab1 siRNA. Then cells were treated with or without BMP2 at 100 ng/ml. RNA was isolated 24 h after BMP2 treatment for RT-PCR as described in “Materials and methods.” Panel A Reduction of Jab1 protein expression in western blot using Jab1-specific antibody. Panel B Increase in BMP-induced ALP mRNA level upon Jab1 siRNA treatment
