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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Osteoarthritis Cartilage. 2011 Jul 29;19(10):1246–1253. doi: 10.1016/j.joca.2011.07.011

Fig. 3. COMP is an early response gene regulated by TGF-β1.

Fig. 3

(A) TGF-β1 signaling pathway is involved in the induction of COMP expression. BMSC grown in monolayer were pretreated with the indicated amount of the specific TGF receptor type I inhibitor SB-431542 for 2 h, followed by stimulation with TGF-β1 for 24 h. The mRNA level of COMP was measured as described in Fig. 1(A). (B) BMSC grown in monolayer were pre-treated with the indicated amount of SIS3 for 2 h, followed by treatment with or without TGF-β1 for 24 h. The mRNA level of COMP was measured as described in Fig. 1(A). (C) Induction of COMP mRNA by TGF-β1 does not involve new protein synthesis. BMSC grown in monolayer were pre-treated with 20 mM Chx for 2 h, followed by the addition of TGF-β1 for 24 h. The mRNA level of COMP was measured as described above (all data shown were means with 95% CIs, n = 3). (D) Parallel BMSC samples from C were lysed in sample buffer and the level of COMP protein was determined by Western blot analysis.