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Comparison of cardiomyocytes nuclear number. Cardiac tissues derived from offspring of control and diabetic groups from E18.5 (white bars), 6 weeks (grey bars), and 12 weeks (bars) of age were stained with haematoxylin and eosin. Slides were digitally scanned and the number of nuclei of the cardiomyocytes was calculated using a modified algorithm from Aperio. Mean nuclear number is presented. *P < 0.05.
