Figure 1. Sequence read distribution across 189 amplicons generated from 238 FFPE specimens, normalized to 300,000 reads per sample.

A. Distribution of average coverage of each amplicon. Data are shown as mean ± SD. B. Number of amplicons with a given read depth, sorted in bins of 100 reads. Blue bars represent number of target amplicons within read depth, red line presents % of target amplicons ≥ read depth.