Table 2. Comparison between wild type and Δmps1 strains with respect to observed phenotypes via DAPI staining.
Time | Strain | Percentage of non dividing cells | Percentage of dividing cells with buds without nuclei | Percentage of dividing cells with normally dividing nucleus | Percentage of mother cell and bud with nuclei | No. of cells counted |
2Hr | Wild Type | 94 | 1 | 1 | 4 | 226 |
MFD2 | 40 | 38 | 7 | 15 | 250 | |
MCM+M/C | 25 | 55 | 8 | 12 | 278 | |
MCM-M/C | 92 | 2 | 1 | 5 | 245 | |
4Hr | Wild Type | 83 | 1 | 2 | 14 | 291 |
MFD2 | 27 | 58 | 6 | 9 | 282 | |
MCM+M/C | 21 | 67 | 8 | 4 | 274 | |
MCM-M/C | 76 | 2 | 3 | 19 | 265 |
Cells were stained with DAPI and counted on hemocytometer. Cells were grown in SD media at 30°C for indicated time points. Average percentages of three biological replicates are shown in the table.