Detection of mRNA expression of NHE isoforms 1–5 in the mouse striatum and ventral midbrain. Striatum (upper panel) and ventral midbrain (lower panel) from mice were dissected for mRNA extraction and RT-PCR detection with specific primer pairs for NHE isoforms 1–5. Lanes 2, 5, 8, 11, and 14 contained reverse transcribed mRNA from each brain region with the respective specific primer sets. Lanes 3, 6, 9, 12, and 15 represent RT-PCR products with the mRNA template and primers in the absence of reverse transcriptase. Lanes 4, 7, 10, 13, and 16 represent primers only. Molecular weight marker is shown in lane 1. Data are representative of two independent experiments.