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. 2004 May 3;101(19):7264–7269. doi: 10.1073/pnas.0400372101

Fig. 1.

Fig. 1.

Single-turnover kinetics of unwinding different lengths of DNA at 2 mM and 200 μM dTTP. The unwinding of ds18 (•), ds30 (▾), ds40 (▪), ds60 (♦), and ds90 (▴) DNAs (Table 1) was measured at 18°C in the presence of a trap at 2 mM (A) or 200 μM (B) dTTP. (A) The data represent an average of three measurements and were globally fit to Eqs. 1 and 2 to obtain s = 9.0 (± 0.7) bp per step, k = 1.7 (± 0.14) steps per s, and Lm = 11.8 (± 0.34) bp. (B) The global fit of the kinetics at 200 μM dTTP provided s = 6.0 (± 0.8) bp per step, k = 1.9 (±0.3) steps per s, and Lm = 11.5 (± 0.5) bp.