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Unwinding processivity of the T7 helicase at 2 mM (A) and 200 μM(B) dTTP. Amplitudes from the global fitting (Fig. 1 A and B) are plotted against corrected duplex length (L - Lm) and fit to Eq. 3 to obtain the processivity of 0.9835 (± 0.0014) at 2 mM dTTP and 0.9703 (± 0.0029) at 200 μM dTTP. The initial fraction of productive helicase-DNA complex was A0 = 0.65 (± 0.02) at 2 mM dTTP and 0.31 (± 0.018) at 200 μM dTTP.
