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. 2014 Jul 15;9(7):e102041. doi: 10.1371/journal.pone.0102041

Figure 2. WT1 regulates Snail expression.

Figure 2

(A) Analysis of Snail protein (top) and mRNA (bottom) in the isogenic SN12C and ACHN cell lines. (B) HEK293T cells were transfected with scrambled oligonucleotides or VHL-specific siRNAs and Snail protein was measured. (C) HEK293T cells were transfected with GFP or GFP-WT1 and Snail protein (top) and mRNA (bottom) was measured. Graphs show mean±SD of one representative of three independent experiments. *, P<0.05. (D) HEK293T and SN12C-VHL cells were transfected as indicated and protein expression was assessed by immunoblot.