Fig. 5.

T7RNAP transcription time course for arrest at T-paired adducts in the transcribed strand. (A) T7RNAP arrest at M₁dG (lanes 1-7) or PdG (lanes 8-14) paired with C in the Xts template was observed for 60 min after the initiation of elongation by NTP addition. Transcripts after a 5-min elongation on the template with dG are in lane 15. RO, runoff transcripts; X, adduct-arrested transcripts. (B) A graph of the % of transcripts arrested at X (•, M₁dG arrest; ▪, PdG arrest) and the % of full-length transcripts (○, RO past M₁dG; □, RO past PdG) for each template over the T7RNAP elongation time course.