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M1dG and PdG block transcript elongation by RNAPII. 32P-labeled transcripts from RNAPII elongation on the Xts (TS) with X opposite C (A, lanes 1-3) or opposite T (B, lanes 1-3) are shown along with transcripts from RNAPII elongation on Xnts (NTS) opposite T (B, lanes 4-6). The base at X is indicated above each lane (G, dG; M, M1dG; P, for PdG). RO, runoff transcripts; X, adduct-arrested transcripts.
