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. 2004 Apr 26;101(19):7281–7286. doi: 10.1073/pnas.0401516101

Fig. 2.

Fig. 2.

Generation of the ChREBP-/- mice. The schematic diagram of genomic mouse DNA is shown for the region surrounding the basic helix-loop-helix-encoding exons (13 and 14) of the ChREBP gene (GenBank accession no. AC084109). The genomic region spanning coding exons 12-14 was replaced with a neomycin-resistance cassette provided by the pKO Scrambler NTKV-1901 vector (Stratagene). Targeted disruption of the ChREBP allele was generated in 129S6/SvEvTac-derived embryonic stem cells with the resulting progeny comprised of 129S6/SvEvTac:C57BL/6J mixed-strain mice. Homologous recombination of the targeting construct at the appropriate site was confirmed by Southern blot analysis (data not shown). Loss of ChREBP expression was confirmed by real-time PCR (Table 2).