Table 2. Relative liver mRNA levels in ChREBP−/− mice fed a standard rodent chow or a high-carbohydrate diet.
| Diet
|
||
|---|---|---|
| Gene | Standard | Starch |
| ChREBP | 0.00 | 0.00 |
| ATP citrate lyase | 0.82 | 0.77 |
| ACC1 | 0.76 | 0.62 |
| ACC2 | 0.62 | 0.49 |
| CPT1 | 0.86 | 0.67 |
| Fructokinase | 0.59 | 0.39 |
| FAS | 0.51 | 0.63 |
| Glucokinase | 2.00 | 1.03 |
| G6P dehydrogenase | 0.97 | 0.84 |
| G6Pase | 0.88 | 0.44 |
| Glut2 | 0.32 | 0.07 |
| Glycogen phosphorylase | 0.71 | 0.57 |
| Glycogen synthase | 0.76 | 0.74 |
| LCE elonelongase CE | 1.19 | 0.58 |
| LPK | 0.27 | 0.12 |
| Malic enzyme | 0.41 | 0.24 |
| PEPCK | 1.66 | 0.80 |
| PFK | 1.00 | 0.74 |
| SCD | 1.00 | 0.30 |
| SREBP-1c | 1.11 | 1.21 |
| SREBP-1a | 0.79 | 0.91 |
| SREBP-2 | 1.08 | 0.98 |
| S14 | 0.98 | 0.61 |
| Triose kinase | 0.35 | 0.25 |
Wild-type and ChREBP−/− mice were housed in individual cages with ad lib access to the diets unless otherwise noted. The standard rodent chow (Harlan Teklad Mouse/Rat Diet 7002) contains ≈30% (wt/vol), starch, 3% sucrose, and 6% fat. The high-starch diet contained 60.2% starch, 20% casein, 1.0% fat, 15% cellulose, and 2.5% vitamin and mineral mix (AIN-76, Harlan Teklad). Mice were fed the high-starch diet for 1 week before death. Equal amounts of liver mRNA pooled from four to five mice of each group, and specific mRNA levels were determined by real-time RT-PCR. The comparative cycle threshold method was used to determine mRNA levels in ChREBP−/− mice relative to age- and diet-matched wild-type mice, which are arbitrarily assigned a value of 1 for each mRNA species. Cyclophilin mRNA level was used as the invariant control.