Table 3. Metabolites in ChREBP−/− and wild-type mouse livers.
| Parameter measured, μmol/g liver
|
Standard diet
|
High-starch diet
|
||
|---|---|---|---|---|
| WT | ChREBP−/− | WT | ChREBP−/− | |
| Glucose | 4.6 ± 0.3 | 6.7 ± 0.2 | 6.0 ± 0.6 | 7.8 ± 0.5 |
| Glu 6-P | 0.13 ± 0.2 | 0.25 ± 0.03 | 0.13 ± 0.01 | 0.31 ± 0.03 |
| PEP | 0.11 ± 0.03 | 0.20 ± 0.08 | 0.11 ± 0.01 | 0.29 ± 0.02 |
| Pyruvate | 0.13 ± 0.027 | 0.043 ± 0.003* | 0.115 ± 0.012 | 0.118 ± 0.009 |
| Pyruvate/PEP | 1.2 | 0.22 | 1.0 | 0.40 |
Wild-type (WT) and ChREBP−/− mice were fed standard rodent chow or the high-starch diet for 1 week. The mice were killed; livers were removed and immediately freeze-clamped between aluminum blocks cooled in liquid nitrogen. The metabolites were measured according to Bergmeyer (16). n = 4; P < 0.05.