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. 2014 Jun 26;2014:943806. doi: 10.1155/2014/943806

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Copyright © 2014 Wouter Eilers et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Isoform specific CaMKII autophosphorylation with repeated contractions in situ. (a)(b) Line graph of the calculated changes in Thr287 phosphorylation of CaMKII isoforms in the oxidative (a) and glycolytic (b) compartment of gastrocnemius medialis muscle as a function of the number of tetanic contractions as paced with repeated trains of 30 pulses of 150 Hz with 4.8 seconds of rest between trains. (c)–(h) Representative immunoblots (c)(d) and bar graphs of mean (e)–(h) of Thr287 phosphorylated CaMKII isoforms in the oxidative (c) (e) (g) and glycolytic (d) (f) (h) compartment of gastrocnemius medialis muscle after 3 and 24 repeated tetanic contractions stimulated via the sciatic nerve with trains of 30 pulses at 150 Hz. ∗denotes P < 0.05 versus rest (ANOVA with post hoc test of Fisher, n = 6).