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. 2004 May 3;101(19):7293–7298. doi: 10.1073/pnas.0401595101

Fig. 3.

Fig. 3.

FRET measurements of DNA bending by GyrA and ParC CTDs. (A) Representative fluorescence spectra of donor-only-labeled 40-bp duplex DNA (yellow), donor plus acceptor-labeled (blue), and donor plus acceptor-labeled plus 10 μM BbGac (green), with maximum donor fluorescence normalized to 1. (B) FRET enhancement by BbGac (green) and E. coli ParC CTD (blue), of the 40-bp DNA substrate. (C) Schematic view of the GyrA CTD (blue and red) bending a 40-bp DNA substrate labeled with donor (D) and acceptor (A) fluorophores, along with approximate distances involved.