Table 2. Primer sets, product size, and GenBank accession numbers for RT-PCR.
| Target gene | GenBank accession no. | Primer sequence forward and reverse (5'—3') | Product size (bp) | Annealing Temp (°C) |
| IL-2Rα | NM_001003211.1 | CAGCCCCCGCTCAAGTGCAT | 207 | 55 |
| GACGCAGCTCGCCACTGCTA | ||||
| IL-4Rα | XM_547077.2 | TGCACGAGCCCAGCTGCTTC | 307 | 65 |
| ACTGTGAGGTTGCCGGGGGT | ||||
| IL-7Rα | XM_850315.1 | ACTCCAGAGATCACAGGGCGGA | 342 | 65 |
| CTGCAAGCCCCCTCCAAGCC | ||||
| IL-9Rα | XM_005621780.1 | GGGCAGGAGCAGCACTATGA | 154 | 65 |
| GCAGCAGAAAGACGGGCACA | ||||
| IL-15Rα | XM_005617203.1 | TGCCTCCCAGGAGACGCCAG | 287 | 65 |
| GCGCCTTCGCTTCAGCCTCT | ||||
| IL-21Rα | XM_844902.1 | GCTCCAGGGAGCCTGGGGTT | 298 | 65 |
| AAAGCTGCCGCACTCCTGGG | ||||
| γc | NM_001003201.1 | TGGGACCGCAGCTGGACTGA | 288 | 55 |
| TCGGGGGATCGACCGTTCCAG | ||||
| γc * | NM_001003201.1 | TACACCCAGGGAACGAAGAGC | 110/114 | 65 |
| GCATGGGGACCGTGGA | ||||
| IFIT1 | XM_843271.2 | TGGCAGTGCAGAGGTCAGGATAG | 119 | 57 |
| GCCAGGTGTATATAAGCAATGTCAA |
*Primer pair used to detect 4-bp deletion in XSCID keratinocytes.