In vitro studies on CSP and COMPcc. a) CD wavelengths scan of CSP (dashed line) and COMPcc (solid line) at 4 °C at 10 μM concentration. Scans represent an average of three trials. b) Plasmid DNA binding to protein with increasing w/w ratio evaluated through mobility shift assay on 1% agarose gel of CSP binding to DNA (left) and COMPcc binding to DNA (right). In both gels L- 1kb DNA ladder, 1- plasmid DNA alone. Proteoplexes prepared at different protein to DNA w/w ratio: 2 – 0.5:1, 3 – 1:1, 4 – 2:1, 5 – 3:1, 6 – 5:1, 7 – 8:1, 8 – 10:1, 9 – 13:1, 10 – 16:1, 11 – 18:1.