Fig. 6.

Flow cytometry analysis of Galα-1,3-Gal epitopes on GGTA1 null piglets and progenitors using BS-I-B₄ lectin. Stained heterozygous or WT cells (red) and unstained cells (black) served as positive and negative controls. (A) Stained (blue) and unstained (black) ear fibroblasts from GGTA1 null piglet O177-1 and fetal fibroblasts from heterozygous progenitor 355-F1 (red). (B) Stained ear fibroblasts from null piglets PL742 (orange), PL743 (blue), and PL744 (green); stained (red) and unstained (black) fetal fibroblasts from heterozygous progenitor 355-F1. (C) Stained (blue) and unstained (black) multilineage white blood cells from O177-1 at 6 weeks of age and stained WBC from an age-matched WT control (red).