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. 2004 Apr 30;101(19):7341–7346. doi: 10.1073/pnas.0308195101

Table 1. RT-PCR primers for allelic expression analysis.

Primers Primer sequence (5′-3′) Product size, bp Location within sequence (GenBank accession no.) Annealing temperatures, °C Conditions, mM MgCl2
P1 AAGAAGCCTCGCCATCACG 112 2080-2192 56 1
P2 GACATGAAGAGGGACTGGTGC (U89358)
P3 ACCAGTCCCTCTTCATGTCAG 289 and 407 2174-2463 59 1.5
P4 AAGATGATCTGAGCACACAAGG (U89358)
P5 AGCGAAGGTTGGGTTTACAA 580 27461-28041 59 1.5
P6 GCCAGGGGCCAAAATATAAG (Z98752)
P7 AAATGGAGATGCTGAGGACACTG 313* 160466-160976 59 1.5
P8 GCCTCTGTCCATTCCAGAAG (AL031681)
*

511-bp PCR product obtained from genomic DNA.