Table 1. RT-PCR primers for allelic expression analysis.
Primers | Primer sequence (5′-3′) | Product size, bp | Location within sequence (GenBank accession no.) | Annealing temperatures, °C | Conditions, mM MgCl2 |
---|---|---|---|---|---|
P1 | AAGAAGCCTCGCCATCACG | 112 | 2080-2192 | 56 | 1 |
P2 | GACATGAAGAGGGACTGGTGC | (U89358) | |||
P3 | ACCAGTCCCTCTTCATGTCAG | 289 and 407 | 2174-2463 | 59 | 1.5 |
P4 | AAGATGATCTGAGCACACAAGG | (U89358) | |||
P5 | AGCGAAGGTTGGGTTTACAA | 580 | 27461-28041 | 59 | 1.5 |
P6 | GCCAGGGGCCAAAATATAAG | (Z98752) | |||
P7 | AAATGGAGATGCTGAGGACACTG | 313* | 160466-160976 | 59 | 1.5 |
P8 | GCCTCTGTCCATTCCAGAAG | (AL031681) |
511-bp PCR product obtained from genomic DNA.