Table 4. Diagnostic potential of individual markers detected in in overnight culture supernatants.
Antigen | Marker | TB | No TB | P- value | AUC % (95% CI) | Sensitivity, % (95% CI) | Specificity, % (95% CI) | Cut off value |
ESAT6/ CFP10 | IP-10 | 16811 (8176–19512) | 1826 (0–19353) | 0.042 | 76 (52–93) | 93 (66–100) | 67 (38–88) | 4499.0 |
ESAT6/ CFP10 | sCD40L | 252 (0–2539) | 0 (0–435) | 0.012 | 78 (61–94) | 78 (49–95) | 60 (32–84) | 15.0 |
ESAT6/ CFP10 | IFN-γ | 339 (8–3578) | 8 (0–1558) | 0.047 | 72 (52–92) | 93 (66–100) | 67 (38–88) | 59.8 |
ESAT6/ CFP10 | TNF-α | 36 (3–169) | 3 (0–113) | 0.033 | 0.7 (50–89) | 93 (66–100) | 53 (27–79) | 4.5 |
ESAT6/ CFP10 | SAP | 4481 (0–22380) | 0 (0–17026) | 0.025 | 74(56–93) | 86 (57–98) | 67 (38–88) | 8.7 |
Rv2029c | IP-10 | 11858 (751–19281) | 3474 (0–18433) | 0.048 | 70 (50–89) | 67 (34–90) | 69 (41–89) | 6805.0 |
Rv2032 | MMP-2 | 0 (0–7236) | 12379 (0–20606) | 0.011 | 83 (2–100) | 100 (78–100) | 75 (35–97) | 9147.0 |
Rv2032 | SAP | 5529 (0–24063) | 0 (0–20031) | 0.023 | 75 (57–93) | 73 (45–92) | 73 (45–92) | 2842.0 |
Rv2389c | CRP | 6317 (0–442665) | 12 (0–116862) | 0.050 | 72 (52–92) | 69 (39–91) | 67 (38–88) | 1922.0 |
Rv2389c | SAP | 6509.0 (0–22648) | 0 (0–10267) | 0.032 | 74 (55–93) | 69 (39–91) | 60 (32–84) | 1216.0 |
Unstimulated | SAA | 705597 (560–4000000) | 15641 (560–309939) | 0.013 | 76 (58–95) | 73 (45–92) | 56 (30–80) | 20389 |
Unstimulated | CRP | 152210 (120–816000) | 30463 (170–816000) | 0.018 | 77 (50–89) | 60 (32–84) | 100 (63–100) | 130552 |
Median levels (and ranges in parenthesis) of analytes detected in overnight (our in-house) whole blood culture supernatants and accuracy in the diagnosis of TB disease. All analyte levels are in pg/ml except for CRP, SAA and SAP (ng/ml). P-values were calculated using the Mann Whitney U test. AUC = Area under the receiver operator characteristics curve. 95% CI = 95% confidence interval. The values shown for the the different antigens were corrected for background by subtraction of the unstimulated levels.