Table 4. Diagnostic potential of individual markers detected in in overnight culture supernatants.

Antigen	Marker	TB	No TB	P- value	AUC % (95% CI)	Sensitivity, % (95% CI)	Specificity, % (95% CI)	Cut off value
ESAT6/ CFP10	IP-10	16811 (8176–19512)	1826 (0–19353)	0.042	76 (52–93)	93 (66–100)	67 (38–88)	4499.0
ESAT6/ CFP10	sCD40L	252 (0–2539)	0 (0–435)	0.012	78 (61–94)	78 (49–95)	60 (32–84)	15.0
ESAT6/ CFP10	IFN-γ	339 (8–3578)	8 (0–1558)	0.047	72 (52–92)	93 (66–100)	67 (38–88)	59.8
ESAT6/ CFP10	TNF-α	36 (3–169)	3 (0–113)	0.033	0.7 (50–89)	93 (66–100)	53 (27–79)	4.5
ESAT6/ CFP10	SAP	4481 (0–22380)	0 (0–17026)	0.025	74(56–93)	86 (57–98)	67 (38–88)	8.7
Rv2029c	IP-10	11858 (751–19281)	3474 (0–18433)	0.048	70 (50–89)	67 (34–90)	69 (41–89)	6805.0
Rv2032	MMP-2	0 (0–7236)	12379 (0–20606)	0.011	83 (2–100)	100 (78–100)	75 (35–97)	9147.0
Rv2032	SAP	5529 (0–24063)	0 (0–20031)	0.023	75 (57–93)	73 (45–92)	73 (45–92)	2842.0
Rv2389c	CRP	6317 (0–442665)	12 (0–116862)	0.050	72 (52–92)	69 (39–91)	67 (38–88)	1922.0
Rv2389c	SAP	6509.0 (0–22648)	0 (0–10267)	0.032	74 (55–93)	69 (39–91)	60 (32–84)	1216.0
Unstimulated	SAA	705597 (560–4000000)	15641 (560–309939)	0.013	76 (58–95)	73 (45–92)	56 (30–80)	20389
Unstimulated	CRP	152210 (120–816000)	30463 (170–816000)	0.018	77 (50–89)	60 (32–84)	100 (63–100)	130552

Median levels (and ranges in parenthesis) of analytes detected in overnight (our in-house) whole blood culture supernatants and accuracy in the diagnosis of TB disease. All analyte levels are in pg/ml except for CRP, SAA and SAP (ng/ml). P-values were calculated using the Mann Whitney U test. AUC = Area under the receiver operator characteristics curve. 95% CI = 95% confidence interval. The values shown for the the different antigens were corrected for background by subtraction of the unstimulated levels.