Table 5. Accuracy of combinations of analytes detected in overnight culture supernatants.
| Resubstitution Classification Matrix | Leave-one-out Cross validation | ||||||||
| Host marker model | % TB cases | % HHCs | Total % | % TB cases | % HHCs | Wilks lambda | f | Error df | P-value |
| TNF-α(Rv2029c), IFN-α2(Rv2032), SAP(Rv2032), IL-15(Rv2389c) | 100 | 85 | 92 | 92 | 85 | 0.799 | 5.0 | 20 | 0.037 |
| SAP(Rv2029c), TGF-α(Rv2032), SAP(Unstimulated), TGF- α(Unstimulated) | 92 | 79 | 85 | 92 | 64 | 0.949 | 1.1 | 21 | 0.302 |
| IL-1α(ESAT6/CFP10), SAP(Rv2029c), TGF- α(Rv2389c), SAP(Unstimulated) | 100 | 79 | 88 | 92 | 79 | 0.909 | 2.1 | 21 | 0.163 |
| fractalkine(ESAT6/CFP10),SAP(Rv2029c), TGF-α(Unstimulated), SAP(Unstimulated) | 92 | 71 | 80 | 92 | 71 | 0.986 | 0.3 | 21 | 0.602 |
| MIP-1β(Rv2029c), IFN-α2(Rv2032), SAP(Rv2032), IL-15(Rv2389c) | 100 | 85 | 92 | 92 | 77 | 0.814 | 4.6 | 20 | 0.045 |
| IL-1α(Rv2029c), SAP(Rv2029c), IL-15(Rv2389c), IL-1 α(Unstimulated) | 92 | 71 | 81 | 75 | 64 | 0.493 | 21.6 | 21 | 0.000 |
| SAP(Rv2029c), fractalkine(Rv2032), TGF- α(Unstimulated), SAP(Unstimulated) | 100 | 86 | 92 | 83 | 71 | 0.910 | 2.1 | 21 | 0.166 |
| sCD40L(ESAT-6/CFP-10),IL-1α(Rv2029c),TGF-α(Rv2032), CRP(Rv2389c) | 92 | 92 | 92 | 92 | 92 | 0.623 | 12.1 | 20 | 0.002 |
| IL-12p40(ESAT-6/CFP-10), IP-10(Rv2032), VEGF(Rv2032), TGF-α (Unstimulated) | 64 | 54 | 59 | 64 | 54 | 0.578 | 16.0 | 22 | 0.000 |
| fractalkine(ESAT-6/CFP-10), IL-1β(ESAT-6/CFP-10), SAP(Rv2029c), TGF-α(Unstimulated) | 94 | 64 | 77 | 92 | 57 | 0.451 | 25.5 | 21 | 0.000 |
| fractalkine(ESAT-6/CFP-10), IL-10(ESAT-6/CFP-10), SAP(Rv2029c), TGF-α(Unstimulated) | 100 | 64 | 81 | 100 | 57 | 0.522 | 19.2 | 21 | 0.000 |
| IL-12p70(Rv2029c), IP-10(Rv2032), VEGF(Rv2032), TGF-α (Unstimulated) | 92 | 36 | 62 | 58 | 29 | 0.632 | 12.2 | 21 | 0.002 |
| MIP-1 α(Rv2029c), IFN- α 2(Rv2032), SAP(Rv2032), IL-15(Rv2389c) | 100 | 77 | 88 | 92 | 77 | 0.862 | 3.2 | 20 | 0.089 |
| sCD40L(ESAT-6/CFP-10),IL-17(Rv2029c), SAP(Rv2032), IL-15(Rv2389c) | 100 | 85 | 92 | 92 | 85 | 0.504 | 19.6 | 20 | 0.000 |
| sCD40L(Rv2029c), IL-12p70(Rv2032), SAP(Rv2032), IL-15(Unstimulated) | 100 | 86 | 92 | 100 | 79 | 0.376 | 34.8 | 21 | 0.000 |
| SAP(Rv2029c), EGF(Unstimulated), TGF- α (Unstimulated), SAP(Unstimulated) | 100 | 67 | 81 | 92 | 67 | 0.965 | 0.8 | 22 | 0.386 |
| IP-10(ESAT-6/CFP-10), IL-12p40(Rv2029c), IP-10(Rv2032), TGF- α (Unstimulated) | 100 | 85 | 92 | 83 | 85 | 0.795 | 5.1 | 20 | 0.035 |
| MMP-9(Rv2029c), IFN- α 2(Rv2032), SAP(Rv2032), IL-15(Rv2389c) | 92 | 100 | 94 | 92 | 100 | 0.752 | 4.3 | 13 | 0.059 |
| IP-10(Rv2032), VEGF(Rv2389c),IP-10(Unstimulated), IL-10(Unstimulated) | 77 | 69 | 73 | 62 | 62 | 0.825 | 4.5 | 21 | 0.047 |
| SAP(Rv2029c), IL-1 β (Unstimulated), TGF- α (Unstimulated), SAP(Unstimulated) | 92 | 73 | 91 | 92 | 73 | 0.985 | 0.3 | 22 | 0.570 |
Accuracy of 4-analyte models generated for markers detected in overnight (our in-house) whole blood culture supernatants in the diagnosis of TB disease. In each case, effect df = 1.