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. 2014 Jul 15;9(7):e102584. doi: 10.1371/journal.pone.0102584

Table 7. Utility of combinations of analytes detected in QFT-IT supernatants in the diagnosis of TB disease.

Resubstitution Classification Matrix Leave-one-out Cross validation
Host marker model % TB cases % HHCs Total % % TB cases % HHCs Wilks lambda f P-value
IL-12p40Ag-Nil, IL-12p70Ag- Nil, IL-15 Nil, IL-10 Nil 100 100 100 100 89 0.850 3.2 0.092
EGFAg, IL-12p40Ag-Nil, IL-12p70 Ag- Nil, IL-15 Nil 100 100 100 100 89 0.844 3.3 0.084
IL-12p40 Ag-Nil, IL-12p70Ag- Nil, IL-15 Nil, IL-1β Nil 100 89 96 100 89 0.601 11.9 0.002
IL-17Ag, VEGFAg-Nil, fractalkine Nil, IP-10Nil 100 100 100 100 89 0.433 23.5 0.000
IL-10Ag, IL-12p40 Ag-Nil, IL-12p70 Ag-Nil, TGF-α Ag-Nil 100 89 96 100 89 0.994 0.1 0.745
IL-17Ag, MCP-1 Ag-Nil, VEGF Ag-Nil ,IP-10 Nil 100 100 100 100 100 0.426 24.2 0.000
IL-12p40 Ag, IL-10 Ag, IL-10 Ag-Nil, IL-12p70 Ag- Nil 100 100 100 100 89 0.812 4.2 0.056
IL-12p40 Ag, IL-12p40 Nil, IL-12p70 Ag- Nil, IFN-γ Nil 93 100 96 93 89 0.886 2.3 0.147
IL-12p40 Ag, IL-10 AgIL-12p70 Ag- Nil, IL-10 Nil 100 100 100 100 89 0.794 4.7 0.045
IL-10Ag, EGF Ag- NilIFN-α2 Ag- Nil, IL-12p70 Ag- Nil 93 100 96 93 89 0.890 2.2 0.152
IL-12p40 Ag, IL-12p40 Ag-Nil IL-12p70 Ag- Nil, IL-10Nil 100 100 100 93 100 0.929 1.4 0.256
IL-1αAg, IL-12p40Ag-Nil, IL-12p70 Ag- Nil, IL-15 Nil 100 89 96 100 89 0.571 13.5 0.002
IL-1αNil, IL-12p40Ag-Nil, IL-12p70 Ag- Nil, IL-15 Nil 100 100 100 100 89 0.667 9.0 0.008
IL-17Ag, EGF Ag-Nil, IL-12p40 Nil, IP-10Nil 93 100 96 93 89 0.371 30.5 0.000
VEGFAg, IL-12p40Ag-Nil, IL-12p70 Ag- Nil, IL-15 Nil 100 89 96 100 89 0.661 9.2 0.007
IL-12p40 Ag-Nil, IL-12p70 Ag- Nil, IL-10 Nil, IFN-γ Nil 93 100 96 93 89 0.921 1.5 0.230
IL-10Ag, MCP-1 Ag- Nil, IL-12p40Ag-Nil, IL-12p70Ag- Nil 100 89 96 93 89 0.995 0.1 0.768
IL-12p40Ag-Nil, IL-12p70Ag- Nil EGF Nil, IL-15Nil 100 100 100 100 78 0.886 2.3 0.147
IL-12p40Ag-Nil, IL-12p70Ag- Nil IL-1βAg, IL-15Nil 100 100 100 100 89 0.760 5.7 0.028
IL-12p40 Ag, IL-12p70 Ag- Nil IL-10Nil, IL-10 Ag- Nil 100 100 100 100 89 0.786 4.9 0.040

Accuracy of 4-analyte GDA models in QFT-IT supernatants in the diagnosis of TB disease. Nil = unstimulated marker levels, Ag = levels detected in antigen stimulated supernatants, Ag-N = Antigen-specific biomarker levels obtained after subtraction of Nil responses. In each case, effect df = 1, error df = 18.