Table 8. Correlation between unstimulated and ESAT-6/CFP-10-specific analyte levels detected in supernatants from the three culture techniques.

	QFT-IT Vs overnight		QFT-IT vs 7-day		Overnight vs 7 day		Fold difference
	r2	P value	r2	P value	r2	P value	QFT vs overnight	QFT vs 7-day WBA
ESAT6/CFP10 IP10	0.63	0.0004	0.60	0.001	0.78	< 0.0001	1.3	4.4
ESAT6/CFP10 MCP-1	0.53	0.004	0.35	0.076	0.20	0.31	0.0005	0.0001
ESAT6/CFP10 IFN-γ	0.85	1.44	0.84	3.96	0.77	< 0.0001	2.5	179
ESAT6/CFP10 EGF	−0.16	0.41	−0.20	0.31	0.29	0.15	1	1
ESAT6/CFP10 MMP-9	0.62	0.002	0.19	0.40	0.19	0.40	1.4	1.3
ESAT6/CFP10 sCD40L	0.05	0.78	−0.15	0.44	−0.01	0.97	8.1	39.1
ESAT6/CFP10 SAP	0.74	< 0.0001	0.06	0.75	0.15	0.43	0.75	15.7
Unstimulated Fractalkine	0.40	0.031	0.42	0.021	016	0.41	0.5	1.1
Unstimulated IFN-α2	0.98	< 0.0001	0.18	0.35	0.16	0.40	2	5.7
UnstimulatedMCP-1	0.07	0.73	−0.07	0.72	0.14	0.47	15	200
UnstimulatedMIP-1β	0.45	0.015	0.17	0.39	0.41	0.03	109	746
UnstimulatedVEGF	0.32	0.096	0.08	0.68	0.26	0.17	1.3	4.7
UnstimulatedIL-1β	0.65	0.0001	0.12	0.55	0.40	0.03	115	115
Unstimulated IL-10	0.63	0.0003	−0.18	0.36	−0.15	0.43	2	5.7
Unstimulated MMP-2	0.73	0.0001	0.41	0.06	0.07	0.76	1	23
Unstimulated MMP-9	0.62	0.002	0.19	0.40	0.19	0.41	2.4	13.5
Unstimulated CRP	0.95	< 0.0001	0.38	0.044	0.40	0.033	1.1	39.8
Unstimulated SAA	1.0	< 0.0001	0.91	< 0.0001	0.90	< 0.0001	0.7	39
Unstimulated SAP	0.74	< 0.0001	0.06	0.75	0.15	0.43	1.2	36.3

Correlation matrices were only computed for analytes that discriminated between TB and no-TB with AUC>0.70 in at least one of the WBA techniques. Analytes with r² values >0.5 and p values <0.01 are highlighted bold. The mean values of the markers in the different culture techniques were compared.