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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2014 May 9;20(8):1252–1257. doi: 10.1016/j.bbmt.2014.05.004

Figure 2. Thawed cGMP grade Ex-NK and FA-NK cells expand in vivo when cultured overnight with IL-2 before adoptive transfer.

Figure 2

Ex-NK and FA-NK products were thawed after cryopreservation in 10% DMSO and transferred to NSG mice either immediately (Thaw/inject) or after overnight culture with IL-2 (O/N IL-2). All mice were subsequently given 6 doses of IL-15 at 5 mcg/dose. (A) Cytotoxic activity of each product is shown as the percent of Cr51 labeled K562 cells lysed when incubated for 4h with each product at the indicated ratios. (B) The number of human CD45+ NK cells in 150 μl of blood collected on day 7 and day 14 after transfer. Results shown are the means ± SEM, n=6 mice per group.