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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2014 May 4;20(8):1089–1103. doi: 10.1016/j.bbmt.2014.04.028

Figure 7. Anti-CD20 mAb Prevents Induction of cGVHD in BALB/c Recipients Given MHC-mismatched C57BL/6 Donor T Cells.

Figure 7

Lethally irradiated BALB/c recipients were injected with 1.25×106 whole spleen cells and 2.5 ×106 TBCD-BM cells from C57BL/6 donors and injected i.v. with either rat IgG or anti-CD20 mAb (40 mg/Kg) the following day. Recipients given TBCD-BM alone were used as controls. (A) Recipients were monitored for clinical GVHD, including body weight change, clinical cutaneous cGVHD score and survival. (n=12). (B–C) Recipients from each group were sacrificed on day 50 and (B) percentage and yield of splenic CD4+ and CD8+ donor T cells (H2Kb+TCRβ+) percentage and (C) yield of CD4+CD8+ thymocytes were measured. A representative staining pattern from each group at each time point is shown from four mice from two replicate experiments.