Figure 4. aPKC is enriched in the detergent-insoluble fraction but decreased in the detergent-soluble fraction.
A, Western-blottings with protein lysates from detergent-soluble (S) and insoluble fractions (I) of non transgenic (NT) and SOD1 (G93A) lumbar spinal cords at P120. The graph represents the ratio of aPKC protein level in the insoluble fraction over the soluble fraction, the I/S ratio being set at 1 in the NT mouse group (n=3 animals/group). B, Western-blotting with protein lysates from detergent-soluble fractions of NT and SOD1 (G93A) lumbar spinal cords at P30 and P90 (n=3–5 animals/group).* p<0.05, Student’s t test.
