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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Dev Neurobiol. 2014 Jan 22;74(8):839–850. doi: 10.1002/dneu.22137

Figure 5. Ryk expression is increased in the motor neurons and the white matter of the ventral lumbar spinal cord of SOD1-G93A mice.

Figure 5

Immunostaining of coronal sections of lumbar spinal cords of non transgenic (NT), SOD1 (WT) and SOD1 (G93A) mice of 30, 60, 90 and 120 days for Ryk (green) and ChAT (red). A, Representative pictures of Ryk/ChAT double immunostaining in P30 NT, SOD1 (WT) and SOD1 (G93A) lumbar spinal cords. Arrows indicate motor neurons double labeled for Ryk and ChAT. B, Quantification of Ryk immunoreactivity in ChAT+ motor neurons (MN) in the ventral lumbar spinal cord at different stages. C, Ryk immunostaining in the white matter of the ventral lumbar spinal cord of P120 NT, SOD1 (WT) and SOD1 (G93A) mice. Arrows indicate increased Ryk immunoreactivity in the ventral white matter of SOD1 (G93A) mice. D, Quantification of Ryk immunoreactivity in the ventral white matter of lumbar spinal cord of NT, SOD1 (WT) and SOD1 (G93A) mice at different stages. E, Colocalization of Ryk with NF-200 in the white matter of the ventral spinal cord of P120 SOD1 (G93A) mice. F, Ryk is expressed in SMI-312+ axons in the ventral roots of P120 SOD1 (G93A) mice. Data represent mean±SEM, n=3–10 animals/group, * p<0.05, ** p<0.01, *** p<0.001, ANOVA with Bonferroni Post-test. Scales bars: 50 µm (A,C,E,F).