Figure 5. Effect of Atf3-Morpholino on Atf3 protein synthesis, Socs3 and Gadd54a gene expression.

(A) Representative Western blot showing reduced Atf3 synthesis in mice treated with Atf3-Morpholino and subsequently subjected to mechanical ventilation in the presence of 80 ppm H₂S. (B) and (C) RT-PCR expression values for Socs3 and Gadd45a normalised to GAPDH. Data represent median of fold change ±SEM for n = 4/group. Analysis of variance (Student–Newman–Keuls post hoc test), *P<0.05 vs. control.