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Primary neurons were treated with or without 2.5β-secretase activity assay. (A) The reaction was followed over a time-course of 120 min. Lines represent mean values of fluorescent signal (relative fluorescence units (RFU)). (B) Data at time point 120 min of the assay from (A) is shown in a graph. Columns represent mean values +/− SEM. n = 3 per group, p<0.05.
