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. 2004 May 5;101(19):7481–7486. doi: 10.1073/pnas.0402328101

Fig. 4.

Fig. 4.

Effect of the D1304N mutation on ectopic paternal MEA allele expression. CaMV:DME-4 and CaMV:DME-5 represent two independently isolated transgenic lines that ectopically express the wild-type DME cDNA (8). CaMV:DME(D1304N)-1 and CaMV:DME(D1304N)-3 represent two independently isolated transgenic lines that ectopically express the mutant D1304N form of DME. Total RNA was isolated from pollen harvested from open flowers, and the approximate level of MEA and DME RNA was determined by semiquantitative RT-PCR. (A) CaMV:DME transgenes activate paternal MEA allele gene expression in pollen. (B) CaMV:DME(D1304N) transgenes do not activate paternal MEA allele gene expression in pollen.