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Glucose-stimulation of MIN6 cells for 2 h does not evoke ER stress. (A) Immunoblots for ER stress markers and γ-tubulin in MIN6 cells glucose-stimulated for 2 h. For control, ER stress was induced with 500 nM Thapsigargin. (B) RT-PCR for detection of Xbp1 spliced variants. The unspliced Xbp1 mRNA (479bp) contains a PstI restriction site. PstI restriction digestion generates fragments of 287 bp and 192 bp.
