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. 2014 May 14;3(5):518–530. doi: 10.1016/j.molmet.2014.05.002

Figure S4.

Figure S4

: Inhibition of the eIF4E/eIF4G binding does not affect glucose-stimulated translation of SG proteins in insulinoma cell lines. (A) Immunoblots for eIF4E, eIF4G and γ-tubulin recovered in cap-binding assays with m7GTP-sepharose beads from extracts of MIN6 cells treated or not with the eIF4E/4G inhibitor for 1 h prior to glucose-stimulation. (B) Immunoblots for SG proteins and γ-tubulin in MIN6 cells pretreated with the eIF4E/4G inhibitor prior to glucose-stimulation. (C) Quantification of SG precursor proteins as detected in (B). (D) Immunoblots for SG proteins and γ-tubulin in MIN6 cells transfected with the m7GpppG cap-analog prior to glucose-stimulation. (E) Quantification of SG precursor proteins as detected in (D) (n = 4). (F) Immunoblots for ICA512, proCgA and γ-tubulin in glucose-stimulated, m7GpppG-transfected INS-1 cells. (G) Glucose-stimulated total protein biosynthesis as measured by 35S-methionine incorporation in m7GpppG-transfected INS-1 (n = 3).