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. 2014 May 20;3(5):565–580. doi: 10.1016/j.molmet.2014.04.010

Figure 6.

Figure 6

Influence of 4-weeks dietary methyl-donor supplementation on genes regulating fatty acid metabolism in the liver. (A) Gene expression of acetyl-CoA carboxylase 1 and 2 (ACC1 and ACC2). (B) Shown are protein expression levels for ACC1 and ACC2 and their phosphorylated form (P-ACC1 and P-ACC2) relative to control in %, and the ratio for P-ACC1/ACC1 and P-ACC2/ACC2 (normalized values were used). Separate gels were run for quantification of total ACC1 and P-ACC1, as well as for ACC2 and P-ACC2. β-ACTIN or HSP90 of the respective membranes were used for protein normalization. Data are presented as mean ± SEM (n = 7–9). Open, gray, open lined and gray lined boxes show data from C, HF, CMS and HFMS animals, respectively. Different subscript letters indicate statistical significance (p < 0.05).