Fig. 4.

Proportions of mature and immature female gametophytes in (A) wt and five HSFB2a overexpressing (35S:HSFB2a, 1–5) and (B) wt and five asHSFB2a (35S:asHSFB2a, 1–5) overexpressing lines. Open boxes represent the proportion of mature female gametophytes, grey boxes the proportion of immature female gametophytes, numbers in and above boxes the respective average percentage of five plants per line. Prevalent aberrant phenotypes of HSFB2a and asHSFB2a overexpressing lines. C Embryo sac with one nucleus. D Embryo sac with four nuclei. Arrow heads depict the position of visible nuclei in the female gametophytes. Text below the pictures indicates the percentage of gametophytes showing the above phenotype in the respective lines